Journal: Pharmaceutics

Article Title: Micellar Form of a Ferrocene-Containing Camphor Sulfonamide with Improved Aqueous Solubility and Tumor Curing Potential

doi: 10.3390/pharmaceutics15030791

Figure Lengend Snippet: Activation and nuclear translocation of the NFkB protein in lung cells. ( A ) Representative immunofluorescent images of A549, H1299, and MRC5 control cells and cells treated with the calculated IC50 concentrations of DK164-NP for 48 h and labeled with anti-NFkB antibody (green). DNA was co-stained with DAPI (blue); ( B ) Quantitative determination of the intensity of the nuclear NFkB-signal, normalized with nuclear DNA content, was performed by CellProfiler cell image analysis software (Broad Institute´s Imaging Platform, Cambridge, MA, USA) and GraphPad Prism v.8.0 (Dotmatics, San Diego, CA, USA). A p -value < 0.05 was considered statistically significant, ** p < 0.01. Quantification is based on three independent experiments with >50 cells scored for each condition. The error bars represent standard deviation (SD). AU: arbitrary unit. Scale bars represent a distance of 15 µm.

Article Snippet: The graphs were generated by GraphPad Prism v.8.0 software (Dotmatics, San Diego, CA, USA) ( B).

Techniques: Activation Assay, Translocation Assay, Labeling, Staining, Software, Imaging, Standard Deviation